Preparation of cytoplasmsic and nuclear fractions with pierce NEPER kit?

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Goodwin G. Jinesh

Wash it in a new/fresh eppendorf. The proteins stuck to the wall of tube could be the source of contamination.

Divaker Choubey

One can be more gentle during the lysis of cells and fractionation into cytoplasmic and nuclear fractions. The nuclear pellet (or any pellet) can be washed with large excess of PBS before proceeding for nuclear lysis. Some contamination (which is acceptable) is unavoidable.