Here's a transfection kit for RAW cells (https://altogen.com/product/raw-264-7-electroporation-kit-lymphoma-cells-tib-71/). Plasmid concentration is usually determined by the molecular weight , but for electroporation what matters most is that your reagent does induce cytotoxicity in the cells.

To my experience, 1-1.5 microgram of total plasmid with a concentration no lesser than 250 nanogram per microliter is standard. Best wishes.

You need to first conduct a cytotoxicity assay to your transfection reagent (TR) alone with the cell lines that you want to transfect so as to find out the highest concentration of the TR that can be used with minimal cytotoxicity.

Then you can follow this protocol:

1- Plate 1–2 * 10^4 adherent cells or 1–2 * 10^4 to 1 * 10^5 suspension cells in 100μl per well of a 96-well plate. Incubate overnight.

2- Allow the TR to warm to room temperature, warm serum free (SF) or low serum (LS) DMEM to 37C and cool to room temperature..

3- Prepare a mixture of the optimum concentration of the TR in SF or LS and 20ng/ul of your plasmid in this order (Medium-TR-Plasmid). Flick to mix between each addition but do not vortex. Incubate for 20mins at room temperature.

4- Add 10ul of the transfection mixture to each well and incubate overnight.

Good luck

Hi, maybe these 2 recent publications can be helpful (use of Viromer RED for plasmid transfection).

The p7 viroporin of the hepatitis C virus contributes to liver inflammation by stimulating production of Interleukin-1 β.

Farag et. al., Biochim Biophys Acta., 2016

Delta-Like Ligand 4 Modulates Liver Damage by Down-Regulating Chemokine Expression.

Shen et. al., Am J Pathol., 2016

Here's a transfection kit for RAW cells (https://altogen.com/product/raw-264-7-electroporation-kit-lymphoma-cells-tib-71/). Plasmid concentration is usually determined by the molecular weight , but for electroporation what matters most is that your reagent does induce cytotoxicity in the cells.