Crispr-cas9 knock out with Mouse cell line?

Hi, anyone have suggestions for crispr cleavage detection using thermo genomic cleavage detection kit? I am trying to make nfkb1 knock out in RAW 264.7 cell line and I have using thermo Cas9...

11 December 2018 4,167 0 View

Get-in fellowship?

hi, anyone can suggest some lab in us working on crisp cell line knock outs?

08 September 2018 8,080 0 View

Gene and protein name nomenclature?

Hi, all.I need to know about gene and protein name nomenclature? Basically as per rules we need to write protein name in capital letters and gene name first letter capitals and rest in small...

08 September 2018 254 0 View

Crispr-cas9 knock out with Mouse cell line?

Hi, anyone has information regarding peoples working on crispr- cas9 in mouse cell lines? I want to apply for GET-in fellowship, so I need to contact person's working on same platform in which I...

04 May 2018 194 1 View

Inos detection after H.pylori treatment in RAW 264.7 macrophages?

Hi all, I need to can we measure i-NOS in h.pylori treated samples collected in PBS? After infection of any cell line , we usually washed cells with PBS before collection of cell pellet for RNA...

04 May 2018 3,931 2 View

Cell counting for time kinetics Experiment?

Hi, I need to know about cell counting for time point experiments- I am working on Raw 264.7 cells which has 11 hours doubling time. I use to seed 1 Million cells in six well plate which has...

31 December 2017 618 3 View

H.pylori 26695 wild type strain?

HI, all I am working on H.pylori strain 26695 since 2 years but now its showing nay increase in il-beta or Inos production when infecting raw cells with same. this is bacteria we can borrowed from...

31 December 2017 5,592 1 View

Transfection protocol for raw cells?

Can anyone has standardized protocol for Transfection in raw cell line? I have Thermo Ltx reagent? Is it vary with plasmid type? I have Pcdna 3with flag and his tag?? please suggest plasmid conc....

31 December 2017 1,194 5 View

License for knock out permit?

Hi, All Can anyone tell me is JNU has license to import knock out mice?

11 December 2017 509 0 View

Preparation of cell line knock out using crisper?

Hi, All, I like to know can anyone tell me or suggest me some labs in India preferably Delhi, who have done knock out in mammalaina cells using crisper technology.

09 October 2017 8,631 0 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View