HI all, can anyone tells me if we want to infect any cell line with bacteria so can we seed cells up to maximum amount we can seed in an dish or less?

like in six well i use to seed 1 million o/n in ab free media and then i infect in morning, taking samples at 6 hour interval til 24 hrs.so, obviously at 24hrs comparison to 6 hrs there will be more growth?

i need to know is it good practice to count cell and then plate on respective dish or first grow itself on the same?

obviously we can seed same amount but if my exp involves time kinetics study, so how much this going to vary my experiments??

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