Hi, I need to know if 26695 produces inos?
I am treating raw 264.7 cells with H. pylori strain and I need to know wheter infection is there, since there is upregulation of il-beta in treated compared to control but their is no NO production as measured by griess reagent kit promega
there is no morphological changes in raw as usually seen in ags cells(hummingbird phenotype)
Take a look at these articles. Apparently, Arginase from H. pylori inhibits NO production (1st article). If you want to confirm the infection you can do a Giemsa stain (2nd article).
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC61129/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1731087/
Hi Andre I need to know can I confirm H.pylori infection with staining after infection i.e at 6,1,18 and 24 hrs post infection? I need to confirm after as before i am checking under microscope before infecting.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology