Is it required that we target all four alleles of a gene in tetraploid potato desiree for knockout studies using CRISPR/Cas9 technology? How effective is CRISPR/Cas9 vector transformation in Desiree? Is bombardment more preferable? If someone has done any research on CRISPR/Cas9 technology on Desiree, kindly let me know.
Most likely, depending on the gene. The Arabidopsis optimized Cas9 has worked well in my experiments (see attached.) Good luck!
In diploid plant, if genotype is AA, then you have to knock out both alleles (become A'A', biallelic mutant) to get a mutant phenotype. Because both AA and AA' (knock out only one allele; mono-allelic mutant) will be wild-type-like.
Similarly, you need to knock out all alleles (AAAA, tetraploidy) potato to produce a mutant line.
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