Kindly let me know if GV3101-pMP90RK Agrobacterium strain works for transformation of Benthamiana.
Dear Sarbesh,
At first you should try with the strain in benthamiana. Then only you will come to know whether it is efficient or not. At the same time do transformation with other strains also and compare the results. Finally you will get to know which strain is efficient.
Dear Sarbesh,
Are you planing a transient expression experiment? In our experience, all the Agro strains we employed worked well with N. benthamiana for transient expression. I suggest you include among your construct, one that works as positive control for the assay, such as a hairpin agains an easy to visualize gene (e.g. Mg2+-chelatase that inhibits the chlorophyll synthesis). Good luck with the experiments. Cheers,
Dear Sarbesh,
transient expression of recombinant proteins in N. benthamiana using Agrobacterium tumefaciens strain GV3101-pMP90RK works very well, but is also depending on your to be utilized vector system. For example, my colleagues and I at the RWTH and the Fraunhofer IME successfully use the pTRA-vector system for more than a decade right now. Which vector system do you want to employ in your experiments?
From international collaborators I do now that not all vector system do have the correct and corresponding (chromosomal / Ti plasmid) features (genetic background, Ti plasmid, genetic marker genes etc.) required for successful transfection / transformation of GV3101. Because of that I'd agree with Prem and Ezequiel concerning a pre-test with different strains to find the best working one for you. If you need additional information on different Agro strains, order numbers etc just let me know.
Due to the fact that I'm originally from Turkey, I'm curious about your project :). What are you going to express?
Best regards,
Güven
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