27 Questions 9 Answers 0 Followers
Questions related from Sammy Pontrelli
Is there a way to buffer osmolarity similar to how pH can be buffered? For instance, lets say I have a solution with a given osmolarity. Then I add salt, which would ideally increase the...
09 September 2019 7,437 6 View
I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...
09 September 2019 4,540 5 View
16s DNA sequencing protocols often entail the use of genomic DNA extraction. The purified DNA is then used as an input for a PCR reaction that amplifies the 16s region. This is then sequenced. Is...
11 November 2018 6,585 6 View
If you have omics data (metabolomics, proteomics, etc) of two or more biological samples over time, what statistical methods might be useful for either visualization or isolating significant...
07 July 2018 1,733 1 View
Casamino acids is a hydrolysate of casein that primarily contains most amino acids or peptides. But are there other small molecules present, even at low concentrations, that remain after all of...
06 June 2018 8,775 5 View
Thanks
04 April 2018 390 2 View
certain genes (singular and plural) are required for microbial growth in certain conditions. How are these genes identified?
04 April 2018 5,470 0 View
The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...
02 February 2018 2,442 3 View
I'm compiling a list
02 February 2018 6,059 5 View
Do you think it's more on the level of nature communications, or scientific reports?
01 January 2018 4,607 16 View
I would like to make a genomic plasmid library (from E.coli). I am planning to shear the genome with restriction enzymes, then ligate it onto a backbone. I need to transform this library into a...
10 October 2017 4,288 1 View
Hey scientists, can you use your creativity to think about any and all effects a mutation might have on an enzyme? I know most are deleterious, but some increase Vmax or lower Km or change...
09 September 2017 5,679 5 View
I have a protein that doesn't have a Vmax --- this means that the activity keeps increasing no matter how much substrate I add. I've seen people report Kcat/Km in this case, even without...
08 August 2017 7,913 12 View
When trying to determine if a molecule is generally unstable or not, what do you look for? Also, is it possible to determine exactly how it will degrade? For example, if I have an aqueous solution...
07 July 2017 7,654 3 View
If a metabolic gene has a synonymous mutation on it, what can that mean? Here are some hypotheses, please add to it if you think of any- 1. It affects RNA stability and degradation 2. It...
07 July 2017 8,494 5 View
I'm doing an enzyme assay, but I'm having trouble stopping the reaction so that I can get proper kinetic data. I can't heat because the substrates are unstable. I tried filtering, but it...
03 March 2017 480 13 View
02 February 2017 3,898 1 View
There are online tools like SIFT, PANTHER, and PROVEAN that take snp inputs and then output whether that mutation will have an effect on the protein function. I have several genome sequences with...
12 December 2016 9,243 3 View
Doesn't it make sense that superbugs would have lowered DNA replication fidelity? It would allow them to gain resistance to anything much quicker. Is there any evidence for this anywhere?
12 December 2016 9,627 2 View
I would like to purify the total DNA contents of my e.coli culture. I want to keep both the plasmids and the genomic DNA. Can someone tell me if there is a kit that can do this for me? Or will I...
04 April 2014 7,488 2 View
I would like to screen through hundreds of colonies for cell growth. Is there an easy way to grow hundreds of different cultures of e.coli in a shaker incubator with limited space? Ideally there...
02 February 2014 9,582 2 View
I would like to put them in a shaker incubator.
02 February 2014 304 3 View
I am trying to purify a new recombinant protein from e.coli. After I purify the protein and run on an SDS gel, I get the correct sized bands. When I run the sample on the FPLC, I get a vary small...
07 July 2013 976 2 View
The instruction manual is telling me not to centrifuge my magnetic GST beads: http://www.piercenet.com/instructions/2162076.pdf However, I don't have a magnetic stand and would like to...
06 June 2013 5,020 1 View
I am doing a protein purification of GST from E.coli cell lysate. I need to purify this protein under naturing conditions using Glutathione Agarose beads. I have no more glutathione. Does anyone...
05 May 2013 6,497 4 View
For many fellowships, postdocs may be given smaller ($10-30,000/year) amounts of independent research funds. Do you have any tips for how best this money can be spent? What is the most...
01 January 1970 718 5 View
There has lately been a lot of attention given to the exploitative nature of large publishers such as Nature, Science, and Cell. They are monopolizing our scientific communities. Moreover,...
01 January 1970 4,826 2 View
