certain genes (singular and plural) are required for microbial growth in certain conditions. How are these genes identified?
I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...
08 September 2019 4,442 5 View
Is there a way to buffer osmolarity similar to how pH can be buffered? For instance, lets say I have a solution with a given osmolarity. Then I add salt, which would ideally increase the...
08 September 2019 7,327 6 View
16s DNA sequencing protocols often entail the use of genomic DNA extraction. The purified DNA is then used as an input for a PCR reaction that amplifies the 16s region. This is then sequenced. Is...
10 November 2018 6,494 6 View
If you have omics data (metabolomics, proteomics, etc) of two or more biological samples over time, what statistical methods might be useful for either visualization or isolating significant...
06 July 2018 1,620 1 View
Casamino acids is a hydrolysate of casein that primarily contains most amino acids or peptides. But are there other small molecules present, even at low concentrations, that remain after all of...
05 June 2018 8,691 5 View
Thanks
03 April 2018 307 2 View
The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...
01 February 2018 2,352 3 View
I'm compiling a list
01 February 2018 5,961 5 View
Do you think it's more on the level of nature communications, or scientific reports?
31 December 2017 4,331 16 View
I would like to make a genomic plasmid library (from E.coli). I am planning to shear the genome with restriction enzymes, then ligate it onto a backbone. I need to transform this library into a...
09 October 2017 4,204 1 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View