certain genes (singular and plural) are required for microbial growth in certain conditions. How are these genes identified?
I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...
08 September 2019 4,578 5 View
Is there a way to buffer osmolarity similar to how pH can be buffered? For instance, lets say I have a solution with a given osmolarity. Then I add salt, which would ideally increase the...
08 September 2019 7,491 6 View
16s DNA sequencing protocols often entail the use of genomic DNA extraction. The purified DNA is then used as an input for a PCR reaction that amplifies the 16s region. This is then sequenced. Is...
10 November 2018 6,635 6 View
If you have omics data (metabolomics, proteomics, etc) of two or more biological samples over time, what statistical methods might be useful for either visualization or isolating significant...
06 July 2018 1,812 1 View
Casamino acids is a hydrolysate of casein that primarily contains most amino acids or peptides. But are there other small molecules present, even at low concentrations, that remain after all of...
05 June 2018 8,815 5 View
Thanks
03 April 2018 419 2 View
The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...
01 February 2018 2,475 3 View
I'm compiling a list
01 February 2018 6,127 5 View
Do you think it's more on the level of nature communications, or scientific reports?
31 December 2017 4,723 16 View
I would like to make a genomic plasmid library (from E.coli). I am planning to shear the genome with restriction enzymes, then ligate it onto a backbone. I need to transform this library into a...
09 October 2017 4,339 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.
04 August 2024 1,973 1 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View