How sensitive is qPCR in CFU/ml?

I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...

08 September 2019 4,442 5 View

Is there a way to buffer osmolarity?

Is there a way to buffer osmolarity similar to how pH can be buffered? For instance, lets say I have a solution with a given osmolarity. Then I add salt, which would ideally increase the...

08 September 2019 7,327 6 View

When sequencing 16s rDNA, is it necessary to first extract genomic DNA before amplification or can the cell lysate be used directly for amplification?

16s DNA sequencing protocols often entail the use of genomic DNA extraction. The purified DNA is then used as an input for a PCR reaction that amplifies the 16s region. This is then sequenced. Is...

10 November 2018 6,494 6 View

What statistical methods might be useful for comparing omics datasets from different groups over time?

If you have omics data (metabolomics, proteomics, etc) of two or more biological samples over time, what statistical methods might be useful for either visualization or isolating significant...

06 July 2018 1,620 1 View

What else is in casamino acids besides amino acids?

Casamino acids is a hydrolysate of casein that primarily contains most amino acids or peptides. But are there other small molecules present, even at low concentrations, that remain after all of...

05 June 2018 8,691 5 View

How can you identify specific defined media that allows growth of newly isolated environmental microbes?

Thanks

03 April 2018 307 2 View

What are some methods for determining if a gene is essential, or essential for microbial growth in a certain environment?

certain genes (singular and plural) are required for microbial growth in certain conditions. How are these genes identified?

03 April 2018 5,364 0 View

What are useful online tools for cloning/genetic modification/molecular techniques?

I'm compiling a list

01 February 2018 5,961 5 View

How good of a journal is Science Advances?

Do you think it's more on the level of nature communications, or scientific reports?

31 December 2017 4,331 16 View

How do I properly pool a genomic plasmid library?

I would like to make a genomic plasmid library (from E.coli). I am planning to shear the genome with restriction enzymes, then ligate it onto a backbone. I need to transform this library into a...

09 October 2017 4,204 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View