Casamino acids is a hydrolysate of casein that primarily contains most amino acids or peptides. But are there other small molecules present, even at low concentrations, that remain after all of the processing? Things like biotin, pantothenate, etc... ?
Good job Nagendra. Sammy, Casamino acids are produced by a controlled acid (HCl or H2SO4) hydrolysis of casein. Apart from amino acids and the ones Nagendra has pinpointed, the hydrolysate also contains small peptides, salt (30-40%), cystine; the oxidized dimeric form of cysteine which serves as a site for redox reactions and a mechanical linkage that allows proteins to retain their 3-dimensional structure (Nelson, D. L.; Cox, M. M.; Lehninger, Principles of Biochemistry. 3rd Ed. Worth Publishing: New York, 2000. ISBN 1-57259-153-6). I hope this was helpful. Cheers!
Hey everyone, thanks for the info. But I still can’t imagine that casamino acids only contains amino acids. Even if the composition is majorly the 19 amino acids, there should still other compounds present that also survived the hydrolysis steps. Things like beta- alanine, homoserine, GABA ....
is this wrong?
Casamino acids is a mixture of amino acids and some very small peptides obtained from acid hydrolysis of casein. It is typically used in microbial growth media. It has all the essential amino acids except tryptophan, which becomes almost destroyed when digested with sulfuric or hydrochloric acid.
Casamino acids is similar to tryptone, the latter differing by being an incomplete enzymatic hydrolysis with some oligopeptides present, while casamino acids is predominantly free amino acids.
Best regards
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