04 April 2018 2 390 Report

Thanks

More Sammy Pontrelli's questions See All
How sensitive is qPCR in CFU/ml?

I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...

08 September 2019 4,442 5 View

Is there a way to buffer osmolarity?

Is there a way to buffer osmolarity similar to how pH can be buffered? For instance, lets say I have a solution with a given osmolarity. Then I add salt, which would ideally increase the...

08 September 2019 7,327 6 View

When sequencing 16s rDNA, is it necessary to first extract genomic DNA before amplification or can the cell lysate be used directly for amplification?

16s DNA sequencing protocols often entail the use of genomic DNA extraction. The purified DNA is then used as an input for a PCR reaction that amplifies the 16s region. This is then sequenced. Is...

10 November 2018 6,494 6 View

What statistical methods might be useful for comparing omics datasets from different groups over time?

If you have omics data (metabolomics, proteomics, etc) of two or more biological samples over time, what statistical methods might be useful for either visualization or isolating significant...

06 July 2018 1,620 1 View

What else is in casamino acids besides amino acids?

Casamino acids is a hydrolysate of casein that primarily contains most amino acids or peptides. But are there other small molecules present, even at low concentrations, that remain after all of...

05 June 2018 8,691 5 View

What are some methods for determining if a gene is essential, or essential for microbial growth in a certain environment?

certain genes (singular and plural) are required for microbial growth in certain conditions. How are these genes identified?

03 April 2018 5,364 0 View

I have a large document, half citations from Mendeley and half from Endnote. How can I merge them?

The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...

01 February 2018 2,352 3 View

What are useful online tools for cloning/genetic modification/molecular techniques?

I'm compiling a list

01 February 2018 5,961 5 View

How good of a journal is Science Advances?

Do you think it's more on the level of nature communications, or scientific reports?

31 December 2017 4,331 16 View

How do I properly pool a genomic plasmid library?

I would like to make a genomic plasmid library (from E.coli). I am planning to shear the genome with restriction enzymes, then ligate it onto a backbone. I need to transform this library into a...

09 October 2017 4,204 1 View

Similar questions and discussions
How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close