I would like to screen through hundreds of colonies for cell growth. Is there an easy way to grow hundreds of different cultures of e.coli in a shaker incubator with limited space?
Ideally there will be at least a half a ml per sample, and I will be able to measure OD simply and quickly.
Any ideas?
Price isn't much of an issue.
For that volume of cells, this can be done in deep-well 96-well plates with shaking. It would require transferring cultures via multichannel pipette into 96-well microplate for OD readings on a plate reader. Alternatively, if you only need OD values and have access to plate readers with incubator/heating and shaking functions (fairly standard on many readers), you can take realtime growth measurements. This allows you to automate the process and take as many OD readings as you would like. Hope that helps.
yes, deep-well plates. covered with air-permeable adhesive film (or adhesive aluminium foil (pierced )) for ventilation. you can find specific clamps to attach to the shaker bed that allow you to pile several plates vertically. since the effective shaking within each well is reduced [by the small well diameter] you may have to increase shaker rpm, length of culture and strength of medium (we've used 2x LB)
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