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Questions related from Sakthivel Govindaraj
Hello Everyone, I am facing trouble to get maximum yield of T cells and NK cells from from female reproductive tract mucosal sampling especially from Cervicovaginal lavage (CVL), please share...
10 July 2021 553 1 View
Hi Everyone, I am facing some issues in brain stereotaxic surgery for bilateral cannula implantation in ventral tegmental area (VTA) region, some animals are dying after surgery. Today, the animal...
09 March 2020 7,599 0 View
Dear all, I have a clarification in the quantification of rat brain Neurotransmitters by HPLC method. How to quantify the neurotransmitters levels from peak area and peak height. Is there...
05 April 2017 5,013 6 View
Hello Dear Friends, I have experienced a problem in the pAKT protein expression, it shows white color band. I have used recently purchased santa cruz antibody, primary antibody...
04 March 2017 1,562 4 View
Hello, I have done a protein estimation by Bradford microplate method by 1:10 dilution of the samples with RIPA buffer. My doubt is, Bradford solution alone will be served as a blank or...
19 February 2017 8,415 5 View
Hello!!! I have done a two-way ANOVA for comparing data of two-time points among three different stress exposure group to compare within and between groups, but the data shows comparison...
25 October 2016 4,585 5 View
Hello to everyone I am working in the field of stress physiology, I am comparing different parameters between Male animals Vs Female animals and within Male and female animals, each group contains...
13 October 2016 5,049 2 View
Hello to everybody!!! I have conducted a questionnaire-based survey study on how prenatal stress linked with offspring's autism spectrum disorder (ASD). I have two groups, I)...
29 June 2016 9,907 3 View
Hello to Everyone, I am working in stress physiology, since corticosterone is the primary biomarker for the stress response. I want to measure cortiocosterone levels in the pregnant...
23 June 2016 4,750 8 View
How to homogenize the rat brain samples for neurotransmitter estimation by using HPLC-ECD. whether we should homogenize by hand using piston or by using homogenizer? if we use homogenizer how...
19 February 2016 2,114 6 View
Which region of Hippocampus in the brain is mostly affected in the offspring by maternal stress in the histopathological studies.
13 January 2016 8,820 4 View
Dear All, How do perform Maternal Odor Preference/Homing Behavior in pups? and how many day old pups should be used for this behavior study??
27 July 2015 7,945 9 View
Hello to everyone!!! I am working in the field of stress physiology, my work is about i will stress to mother during pregnancy and will check cognitive functions of its offspring.. I will using 40...
09 June 2015 9,502 6 View
How BDNF regulates the Wnt2 and shank protein in autism
03 April 2015 1,516 5 View
Thanks in advance for your replies.
12 February 2015 8,840 7 View
For doing biochemical studies in blood which is best Plasma or Serum? ex antioxidants and corticosterone estimation studies
04 January 2015 4,559 11 View
What is the best route of drug (pure chemical compound from marine isolate) administration to increase the bioavailability in brain for long term (25 days treatment) to rat.
08 December 2014 5,860 12 View
In some studies they used sodium valproate and some studies they used valproic acid, so which is the more appropriate chemical compound to induced autism in rat? WIll all the offspring have the...
18 November 2014 2,879 3 View
Is that right methodology that recording EEG and ECG in anesthetized rat? which is the best anesthetic agent to use for electrophysiological recording in rat?
12 November 2014 7,008 4 View
Which is the best anesthetic agent to use for electrophysiological recording namely EEG and ECG?
12 November 2014 775 9 View
What is the relation between delayed eyelid opening and brain development in rats? How can I correlate delayed eyelid opening with brain circuit development?
12 October 2014 5,730 3 View
Learning new things during gestational period how will influence on offspring and what would the possible mechanism of passage of this same information from mother to offspring? Learning new...
27 September 2014 5,603 3 View
If one trained an animal to a particular behavioral experiment, that same animal can use it for learning another experiment. I am not going to do any behavioral assessment in the same animal just...
25 September 2014 513 9 View
Maternal intelligence will transmit to offspring during gestation by epigenetically or by maternal experiences. if suppose intelligence is transmitted via germ cell then how maternal experiences...
24 September 2014 1,649 6 View
What is the relationship between visual perception and stress, anxiety? What is the mechanism visual perception causes the these effects?
21 September 2014 6,112 15 View
Hi.. What is the gestational length of normal Wistrar rats and will this be affected by any environmental conditional? what is the healthy newborn pups body weight?
10 September 2014 8,482 11 View
General anesthetic dose of Ketamine - 80mg/kg & Xylazine - 10mg/kg will affect heart rate of rat? anyone will experienced ECG recording using this anesthetic combination and how much heart...
09 September 2014 2,814 5 View
I have recorded the EEG signals in rat, I have got only two waves one is parietal and occipital waves. Is it good enough to analyze and interpret only with these two waves? how to analyse these data?
06 August 2014 6,426 4 View
During the animal training behavior test like Eight arm radial maze, I used to wear gloves during animal training some of my friends suggest that don't use gloves, if you gloves the animal doesn't...
15 July 2014 8,185 4 View
How do we analyse the EEG recording waves in rat? What are the conclusions we can get from them? For a rat, we are getting only two waves ie frontal waves and occipital waves. How do we analyse...
02 July 2014 5,979 4 View
I am using a drug to reduce a neurodegenerative condition, a drug which is 40% lipid soluble and 60% water soluble so I want to synthesize biodegradable nanoparticles to attach the drug across...
19 June 2014 5,136 3 View
Could anyone suggest a serum or brain cognitive biomarkers and how to test for them in rats? I think N-acetylaspartate is one among the biomarkers in the brain.
14 May 2014 5,512 3 View
While using ketamine xylazine combination of anesthesia, will it affect the EEG recording of brain wave in the rat?
10 May 2014 7,488 15 View
Can anyone shed the light on which is the exact molecular signalling pathway (molecules) involves in psychological stress induced cognitive impairment in rat brain.
15 February 2014 8,415 3 View
Can anyone suggest any available behavioral tests to check the following cognitive domains: i) Knowledge ii) Comprehension iii) Application iv) analysis v) Synthesis vi) Evaluation to test...
15 November 2013 7,086 3 View
I have trouble in preparing liposome nanoparticle for cross-blood brain barrier drug delivery. So, would you please guide me on how to prepare liposome nanoparticles?
23 September 2013 835 8 View
I have gone through many research articles for nano particle synthesis to across blood brain barrier (BBB) drug delivery. Finally i came to know lipid nano particles better and has less adverse...
17 September 2013 2,557 1 View
Can anyone suggest me which is best rat model for neurological studies (especially cognitive, learning and memory studies) either wistar albino rats or sprague Dawely rats? and what are the...
13 September 2013 7,286 29 View
I have done a pilot study with Dieckol compound, which is obtained from Ecklonia cava, a seaweed, seen only in the place in Japan, Korea. In order to continue the core work, I have inquired with...
12 September 2013 8,826 0 View
