Where can I buy proteins?

More Sachin Khiste's questions See All

Do tissues homogenization destroys cell wall ?

Hello Guys, Im doing a compartmental protein extraction for cytoplasm, nuclear and cell wall proteins . In the protocol I have to use beads based homogenizer. Do these homogenizer destroys cell...

08 September 2018 1,598 5 View

What are best way to do immunoprofiling ?

We are looking for different immunocells in cancer research. Can anyone please suggest some current methods, markers fir immunoprofiling.

10 November 2017 8,366 4 View

What are markers for T- cell and B-Cells ?

I have to isolate different T-cell and B-Cells population from mice tumors. Can anyone please suggest me markers for flow cytometry and immunohistochemistry.

10 November 2017 759 9 View

How to extract extracellular proteins from tissues ?

How I extract only extracellular proteins from tissues.

09 October 2017 10,012 3 View

What happens to your signal in a western blot if you don't use saturating amounts of antibody?

Just had this random question, please help.

05 June 2017 4,802 2 View

Need help with Pharmacokinetic analysis, is my study compartment or non-compartmental ?

Hello Guys, Im working on some of the pharmacokinetic parameters of our new drug. Our lab is not PK lab, so I had few basic PK questions. I have inject single dose my compound in mice IP and...

31 December 2016 2,635 9 View

Can it be possible that both the DNA copies of a gene are express into protein?

Hello Everyone, I'm working on a heterozygous gene. It have dominant mutant and recessive wide type. So I just had question that can both of these copies can be expressed into...

10 November 2016 2,140 3 View

Why Agilent HPLC pump not building pressure??

Hello Everyone, I'm working with Agilent 1220 LC system. The openlab software shows that the pump is idle but its shows the pressure is zero. It looks like pump is not building pressure. Can...

09 October 2016 9,533 9 View

What type of solvent are better to analyze sphingolipids i.e ceramide, gb3, lacCer etc by HPLC ??

Hello everyone, I have to develop a method to analyze sphingolipids by HPLC. I'm little confused about which solvents can I use for better results.Can anyone please suggest. Thanks, Sachin

09 October 2016 2,515 3 View

Why two TEM images of same nanoparticles are different ??

Hello Guys, I'm doing the TEM of the nanomicelles. I got two different type of images from same sample and from same grid. One sample haves dark background while other haves bright background....

06 July 2016 4,082 7 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View