Just want to know what is this assay and how its done.
Thanks
Hello Guys, Im doing a compartmental protein extraction for cytoplasm, nuclear and cell wall proteins . In the protocol I have to use beads based homogenizer. Do these homogenizer destroys cell...
08 September 2018 1,598 5 View
We are looking for different immunocells in cancer research. Can anyone please suggest some current methods, markers fir immunoprofiling.
10 November 2017 8,366 4 View
I have to isolate different T-cell and B-Cells population from mice tumors. Can anyone please suggest me markers for flow cytometry and immunohistochemistry.
10 November 2017 759 9 View
How I extract only extracellular proteins from tissues.
09 October 2017 10,012 3 View
Just had this random question, please help.
05 June 2017 4,802 2 View
Hello Guys, Im working on some of the pharmacokinetic parameters of our new drug. Our lab is not PK lab, so I had few basic PK questions. I have inject single dose my compound in mice IP and...
31 December 2016 2,635 9 View
Hello Everyone, I'm working on a heterozygous gene. It have dominant mutant and recessive wide type. So I just had question that can both of these copies can be expressed into...
10 November 2016 2,140 3 View
Hello Everyone, I'm working with Agilent 1220 LC system. The openlab software shows that the pump is idle but its shows the pressure is zero. It looks like pump is not building pressure. Can...
09 October 2016 9,533 9 View
Hello everyone, I have to develop a method to analyze sphingolipids by HPLC. I'm little confused about which solvents can I use for better results.Can anyone please suggest. Thanks, Sachin
09 October 2016 2,515 3 View
Hello Guys, I'm doing the TEM of the nanomicelles. I got two different type of images from same sample and from same grid. One sample haves dark background while other haves bright background....
06 July 2016 4,082 7 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
