I am adding 0.1 or 0.2 mg/ml concentration of SPIONs to MCF 7 cells, and 6 and 9 well plates for 2 different assays. But I don't know what volume of these nanoparticles I have to add. Can anyone help?
We have very little information to work with here. I don't believe anyone can answer this question with the details available.
If you're studying toxicity (or, indeed, almost anything) you should at least do your pilot expts with a range of concentrations. What made you choose 0.1-0.2 mg/ml in the first place?
Did you synthesise the particles, or are they commercial? If they've been used with cells before, look at what concentration was used then, but bear in my mind whatever specific question YOU are trying to address.
What analyses are you performing? If microscopy, you probably need to keep the dose low enough to distinguish cell-associated particles from unattached particles - too high a concentration and everything will be indiscriminately coated.
Are MCF7 cells cultured as adherent cells or in suspension?
Does mg/ml refer to mass by iron, or overall particle mass?
What diameter/volume are the particles? 0.1 mg/ml could be 1 million particles or 50 billion.
If your cells are adherent and your particles sediment, then you need to think in terms of particles/cm2, not particles/ml, and ensure your 6 well versus 9 well expts are equivalent.
Are the particles functionalised? If they're intended for drug delivery, how many do you need to produce a therapeutic effect? If they're intended for MRI, how much intracellular iron do you need to detect labelled cells? I'd suggest you still need to try a range of concentrations in order to determine what concentration gives you a desired outcome, but you at least need to define the goal.
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