20 Questions 35 Answers 0 Followers
Questions related from Najib Abdellaoui
hello I would like to ask for help regarding calculation of dilution factor to get the amount of mycotoxin per gram of sample. the extraction protocol as fellow: -ground 5 g of sample with 25 mL...
30 November 2020 2,091 15 View
hello, I am doing southern blot experiment and I need the blocking reagent from roche or sigma. however, it is not possible to order the reagent from sigma. what is the possible substitute for the...
10 August 2020 2,191 3 View
hello, I am working on the extraction of trichothecenes from the grain of wheat and I would like to ask how is it possible to calculate the concentration of toxin ug per gram of wheat? the...
18 March 2020 7,684 3 View
hello i am working on chlorella vulgaris and i am trying to extract RNA from it. recently i did extraction from 50 ml of chlorella culture using nitrogen liquid to ground the cells then i used the...
13 February 2019 7,974 3 View
hello i wanna design a guide RNA against a gene in zebrafish and i wanna use the RGR system (ribozyme-guide-ribozyme) as in this paper...
30 March 2017 9,057 0 View
hello i am trying to do northern blot for small RNA (microrna) and i didn't do northern blot before. can someone tell me a detailed protocol to detect small RNA using Denaturing Polyacrylamide Gel...
07 March 2017 7,274 3 View
hello i was looking in reference for IPS in zebrafish however i couldnt find someone who did this work in zebrafish. can someone tell me what the reason behind that even the zebrafish is model...
13 February 2017 1,066 4 View
hello I like to know if possible which molecular methods to use to make higher tiger of recombinant vesicular stomatitis virus? thanks for your help.
09 September 2016 1,321 3 View
hey I was wondering if it is possible to use HDV ribozyme at the 5' end instead of hammerhead ribozyme? and how to design the 5' end for that purpose. thanks.
31 August 2016 2,148 3 View
hello I wanna know if it is possible to analyse RNA-seq locally on Linux because of lack of cluster computer and is it possible to do de novo assembly becayse of lack of genome for my specie? if...
01 March 2016 2,639 2 View
hello I am trying to target a gene using artificial microrna technology. I am planing to put the artificial microrna on the 3'utr of EGFP, the transcription will be done using cmv promoter and...
10 January 2016 4,062 3 View
hello everyone I wanna predict the promoter of immediate early gene of herpes virus in carp. I want to know what is the best program for viral promoter prediction? how is it possible to chose the...
22 December 2015 2,868 3 View
hello i got a set of target genes of microrna and i want to do GO enrichment analysis and KEGG pathway analysis. it is possible to use Blast2Go through blast, mapping and annotation? thanks a lot...
22 November 2015 8,217 9 View
hello i did NGS on olive flounder and i got a list of microrna for different time point. now i need to do the target prediction of these microrna. the problem is the olive flounder don't have a...
27 August 2015 1,183 2 View
hello i have NGS data of microRNA at different time with 0 hour as control. the library construction and data analysis was done by a company. i want to know how to normalize the data?
10 August 2015 3,125 5 View
Hello I am doing an experiment on fish and i want to isolate RNA from spleen. I tried different kit (qiagen, geneall) but when I check the RNA quality on TAE gel i get a degradation of RNA. you...
26 June 2015 535 4 View
hello i want to check the RNA integrity using agarose gel. to prepare the gel i will make 1% agarose into TAE after melting and cooling down i will add UltraView - Nucleic Acid Staining Solution...
05 June 2015 505 10 View
hello i am trying to do qPCR for a specific microrna from a virus and i want to confirm the mature microrna and also the pre-microrna. so i want to know how to designer primer to amplify the...
20 March 2015 1,693 1 View
I like to profile circulating microRNA from serum using NGS after exposition of animals to a toxic. For the extraction of microRNA from serum I am going to use QIAGEN mirneasy serum/plasma kit and...
07 February 2015 4,182 4 View
hello i wanna know if it is possible to know the free energy for a given sequence (as example EGFP sequence) and if there is a software to calculate it.
09 December 2014 7,264 1 View
