11 Questions 24 Answers 0 Followers
Questions related from Najib Abdellaoui
hello I would like to ask for help regarding calculation of dilution factor to get the amount of mycotoxin per gram of sample. the extraction protocol as fellow: -ground 5 g of sample with 25 mL...
30 November 2020 1,943 15 View
hello, I am working on the extraction of trichothecenes from the grain of wheat and I would like to ask how is it possible to calculate the concentration of toxin ug per gram of wheat? the...
18 March 2020 7,571 3 View
hello i am trying to do northern blot for small RNA (microrna) and i didn't do northern blot before. can someone tell me a detailed protocol to detect small RNA using Denaturing Polyacrylamide Gel...
07 March 2017 7,205 3 View
hello I am trying to target a gene using artificial microrna technology. I am planing to put the artificial microrna on the 3'utr of EGFP, the transcription will be done using cmv promoter and...
10 January 2016 3,988 3 View
hello i got a set of target genes of microrna and i want to do GO enrichment analysis and KEGG pathway analysis. it is possible to use Blast2Go through blast, mapping and annotation? thanks a lot...
22 November 2015 8,156 9 View
hello i have NGS data of microRNA at different time with 0 hour as control. the library construction and data analysis was done by a company. i want to know how to normalize the data?
10 August 2015 3,042 5 View
Hello I am doing an experiment on fish and i want to isolate RNA from spleen. I tried different kit (qiagen, geneall) but when I check the RNA quality on TAE gel i get a degradation of RNA. you...
26 June 2015 481 4 View
hello i want to check the RNA integrity using agarose gel. to prepare the gel i will make 1% agarose into TAE after melting and cooling down i will add UltraView - Nucleic Acid Staining Solution...
05 June 2015 432 10 View
hello i am trying to do qPCR for a specific microrna from a virus and i want to confirm the mature microrna and also the pre-microrna. so i want to know how to designer primer to amplify the...
20 March 2015 1,622 1 View
I like to profile circulating microRNA from serum using NGS after exposition of animals to a toxic. For the extraction of microRNA from serum I am going to use QIAGEN mirneasy serum/plasma kit and...
07 February 2015 4,118 4 View
hello i wanna know if it is possible to know the free energy for a given sequence (as example EGFP sequence) and if there is a software to calculate it.
09 December 2014 7,195 1 View
