How make higher titer of recombinant virus?

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Nagarjun Kasaraneni

Using transfection ratio of 2:2:1 (HIV gag: gene: VSVG Env) into 293T cells, we were able to achieve higher titer.

Najib Abdellaoui

thanks a lot for your help.

Divya gopinath

When we used 70% cell confluency we were able to achieve higher titer. And moreover you can always grow on large plates and concentrate using amicons (upto 2 folds)