Any suggestions about a pre-microRNA primer design?

20 March 2015 1 2K Report

hello i am trying to do qPCR for a specific microrna from a virus and i want to confirm the mature microrna and also the pre-microrna. so i want to know how to designer primer to amplify the pre-microrna?

Najib Abdellaoui

thanks for your answer. for the mentioned article, i already read it before but i need protocol to design primer using SYBR for detection of pre-microrna.

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