hello
i am trying to do northern blot for small RNA (microrna) and i didn't do northern blot before.
can someone tell me a detailed protocol to detect small RNA using Denaturing Polyacrylamide Gel as first step, after that how to do the norther blot for small RNA.
thank you a lot for your help.
If you look in this paper, it has a protocol.
Fang, P-Y, Gómez Ramos, LM, Holguin, YS, Hsiao, C, Bowman, JC, Yang, H-W, and Williams, LD (2016) "Functional RNAs: Combined Assembly and Packaging in VLPs", Nucleic Acids Res. 10.1093/nar/gkw1154.
thanks prof.loren, howver there isnt any detailed protocol for detecting small rna using northern blot.
The assay is relatively easy.
Check the protocol in:
Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T (2001) Identification of novel genes coding for small expressed RNAs. Science 294:853–858.
The Tuschl lab has published the best detailed protocols for miRNA extraction, detection and sequencing. Check all their publications on the subject.
Best of luck, Catherine Adamidi
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