hello,

I am working on the extraction of trichothecenes from the grain of wheat and I would like to ask how is it possible to calculate the concentration of toxin ug per gram of wheat?

the protocol is as follow:

  • grind 2 g of sample in 15 ml of solvent.
  • filter and transfer 3 mL to the glass tube.
  • pass it through MycoSep 113.
  • remove 1.5 mL and evaporate to dryness.
  • redissolve in 400 uL of the mobile phase.
  • injection of 50 uL and detect using HPLC.

so how to calculate the concentration of mycotoxin per gram of sample?

thank you.

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