hello

I would like to ask for help regarding calculation of dilution factor to get the amount of mycotoxin per gram of sample. the extraction protocol as fellow:

-ground 5 g of sample with 25 mL of methanol: ACN: water (25:25:50).

-shake for 20 min and centrifuge for 10 min at 2500 xg.

-filter supernatant through filter paper.

-take 10 mL of extract, mix with 40 mL of PBS and filter through microfiber filter.

-pass 10 mL of diluted extract through Vicam column, wash column using 10 mL of PBS, and elute fumonisins by passing 1.5 mL of HPLC grade methanol. collect the elute in vial.

-dry down methanol elute.

-redissolve elute in 400 uL of ACN: water (50:50).

-mix 30 uL of well-mixed redissolved elute with 30 uL OPA reagent.

-inject 30 uL.

how to calculate the dilution factor for this protocol to get amount of mycotoxin per g of sample?

thanks for your help.

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