hello

i am working on chlorella vulgaris and i am trying to extract RNA from it. recently i did extraction from 50 ml of chlorella culture using nitrogen liquid to ground the cells then i used the trizol to extract the RNA. after elution using 50 ul of RNAse free water, i removed genomic DNA from the sample using Turbo DNase kit for 30 min at 37 C.

i checked the integrity of RNA using gel electrophoresis and i got this results, i loaded 1 ug of RNA.

my question, i got a clear band of 28S and 18S RNA, i think the third band could be a partial degradation of RNA.

thank you for your input. and what it is the solution to have a better results?.