Can we judge the quality of scientific journal by its impact factor alone?

What are other good indicators of quality journal apart from high impact factor

10 November 2018 7,793 24 View

How to prepare bacterial culture for Scanning Electron Microscope (SEM) study?

I want to prepare lactic acid bacteria from SEM study

31 December 2017 2,128 3 View

How to save Excel graph as image with 300 DPI?

08 September 2017 1,304 43 View

How to prepare 20 mM sodium acetate buffer (pH 4.6)?

How to prepare 20 mM sodium acetate buffer (pH 4.6)

06 July 2017 8,151 4 View

How to prepare 100 ml HCL-KCL 10 mM buffer?

How to prepare 100 ml HCL-KCL 10 mM buffer, pH 4.6Will there be a change in pH after autoclave?

04 May 2017 9,123 2 View

How to add Tertiary Vertical axis in Line Graph?

I want to add tertiary vertical axis in line graph as seen in attached picture

04 May 2017 4,296 4 View

Does Journal Impact Factor Really Matters when getting PhD?

Does Journal Impact Factor Really Matters when getting PhD?

31 December 2016 7,727 6 View

Can I get Gene accession number by submitting single strand 16S rDNA sequence to NCBI or Double strand 16S rDNA sequence is required for this purpose?

Can I get Gene accession number by submitting single strand 16S rDNA sequence of bacteria to NCBI or Double strand 16S rDNA sequence is required for this purpose?

31 December 2016 9,910 6 View

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree?

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree

31 December 2016 4,830 9 View

How long prepared PBS 7.0 (Phosphate Buffered Saline) can be stored?

09 October 2016 4,108 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View