Can we judge the quality of scientific journal by its impact factor alone?

What are other good indicators of quality journal apart from high impact factor

10 November 2018 7,793 24 View

How to prepare bacterial culture for Scanning Electron Microscope (SEM) study?

I want to prepare lactic acid bacteria from SEM study

31 December 2017 2,128 3 View

How to save Excel graph as image with 300 DPI?

08 September 2017 1,304 43 View

How to prepare 20 mM sodium acetate buffer (pH 4.6)?

How to prepare 20 mM sodium acetate buffer (pH 4.6)

06 July 2017 8,151 4 View

How to prepare 100 ml HCL-KCL 10 mM buffer?

How to prepare 100 ml HCL-KCL 10 mM buffer, pH 4.6Will there be a change in pH after autoclave?

04 May 2017 9,123 2 View

How to add Tertiary Vertical axis in Line Graph?

I want to add tertiary vertical axis in line graph as seen in attached picture

04 May 2017 4,296 4 View

Does Journal Impact Factor Really Matters when getting PhD?

Does Journal Impact Factor Really Matters when getting PhD?

31 December 2016 7,727 6 View

Can I get Gene accession number by submitting single strand 16S rDNA sequence to NCBI or Double strand 16S rDNA sequence is required for this purpose?

Can I get Gene accession number by submitting single strand 16S rDNA sequence of bacteria to NCBI or Double strand 16S rDNA sequence is required for this purpose?

31 December 2016 9,910 6 View

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree?

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree

31 December 2016 4,830 9 View

How long prepared PBS 7.0 (Phosphate Buffered Saline) can be stored?

09 October 2016 4,108 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View