I want to know whether I can determine the enzymes GPT, GOT (by GOT and GPT KIT) and total protein (by Lowry method) from fish liver and muscle dried at 60 C, or do I just use fresh tissues?
For any enzymatic assay, the fresh tissue samples should be preferred for getting accurate activity measures. It is difficult to assay the enzyme activities in dried samples. Total protein can be estimated in supernatent of homogenized dried samples with appropriate buffer or NaoH , but the moisture content of the samples before drying is very much important for arriving the exact values.
only fresh tissues that you can used. enzymes couldnot be stable in this condition, also at 60 C protein couldnot be detect as temperture effect on it
I agree with Dr. Ahmed. Micro Kjeldhal method is preferable method for estimating the protein in dried samples. Lowry's method can only be useful for estimating the total soluble protein.
Dehydrated tissues can not be used to estimate enzymes activity. As the drying leads to Denaturation of the proteins and thus lost the enzymes their function .on the other hand, plasma total nitrogen can be estimated and from it we could calculate the plasma protein.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Proteins