Main problem will be collection of blood if you need repeated sampling from the same fish. Estimation of lipid profile in serum is not difficult.

S.Dey

Mehjabeen, are you proposing a study on commercially grown or wild fish? In a particular body of water or just in general? On the latter, if not a real world setting the research may or may not contribute to the literature in a meaningful way. In fact, to be useful, my thought is that you will need to narrow down to a species and specific type of environment.

it is not difficult and good idea.we have done on bone metabolism and published results in j of fish physiology.Contact [email protected] my post graduate student

Mehjabeen,

I routinely measure triacylglyercols, cholesterol, and the enzyme lipase in fish plasma with an auto-analyzer called a VetTest. It is limited in that these are the only lipid related parameters available. I have not tested the effects of metals; however, if growth is altered you may see metabolic disruption and responses in these plasma lipids. Please let me know if you have any questions on this approach. Below are two papers that contain data for fish plasma.

Meador JP et al. 2011. Tributyltin and the obesogen metabolic syndrome in a salmonid. Environmental Research 111:50-56.

Meador JP et al. 2006. Altered growth and related physiological responses in juvenile chinook salmon (Oncorhynchus tshawytscha) from dietary exposure to polycyclic aromatic hydrocarbons (PAHs). Canadian Journal of Fisheries and Aquatic Sciences 63:2364-2376.

The concentration of heavy metals in different fish species have difference eco-toxicological effect, and also, in many papers' results reveal that the adverse effect in one fish tissue have different distribution, such as in fish head, liver, kidney , muscle, and fatty acid , and blood. If you want to study the serum lipid profile of fish, you should be take more account for the field/ or lab experiment to collect the blood samples. It's hard work. In Hygiene, our body's trace element analysis that using blood samples from test persons.

I am concerning with one water body which is polluted by thermal power plant containing heavy metal and the concerned fish is Channa punctatus. It is purely a field work. So doing serum lipid profile is it meaningful?

Dear Mehjabeen,

From Channa punctatus you will get very less quantity of serum. And I do agree with Xiewen Xiao Channa is live fish and lives in hard condition. You have to collect blood sample from different ecological conditions . It will work but it is hard work. I did serum biochemistry test of Catla.

Sir, i did serum profiling but unable to relate with my studies.

while acknowledging the fact that there are many studies on lipid profiling (fatty acid, cholesterol, oxysterol, phytosterol and phospholipid content) in response to toxicants, heavy metals, etc., you could expand your idea on testing antioxidant enzymes, which are directly associated with lipid profiling. Some pesticides such as dicofol increased oxidative stress by elevation of lipid peroxidation index associated with depletion in glutathione level. Cu intake at high concentration induced adverse effects on lipid profile, associated with oxidative stress and diminished activities of antioxidant enzymes.

Dear Mehjabeen Javed,

This is very good study, before that you have decide what heavy metal your going to induce and the fish species at what stage i.e. fry, fingerlings or adult.

You can analyse Lipid Profile, fatty acid profile, Amino acid profile, steroid hormone profile and enzyme assay in serum and also in milt or egg in adult fishes.

I am also doing this type of research induced with radiation.

You can analyse with GC-MS and separate kits for different parameters.

I wish you all the best for your innovative research work.

with warm regards

Prof. M. Saiyad Musthafa