17 Questions 15 Answers 0 Followers
Questions related from Mehar Khalil
What is wrong with my real-time PCR results?
23 May 2023 7,334 3 View
I am getting a thin white layer in my lymphocyte culture. I used dmem media with 10% fbs to culture lymphocytes. After culturing 24 hours in CO2 incubator, i observed a thin white layer formation...
15 March 2023 6,597 1 View
Can I use mem supplemented with fbs to grow lymphocytes. Would this potion be suitable for their growth.
10 March 2023 3,502 3 View
I’m culturing lymphocytes (isolated from whole blood) in RPMI-1640 media supplemented with 10%FBS. After overnight incubation in CO2 incubator at 5% CO2, the media had turned Cloudy and in...
28 February 2023 222 1 View
I performed MTT assay for which I cultured lymphocytes in a 96-well plate. I incubated the cells (lymphocytes) with my test substance for 24 hours, after which I added 2ul of MTT reagent to each...
26 February 2023 2,088 4 View
I‘m trying to culture lymphocytes. I’m attaching the pictures of the media that are available to me. kindly help me out. How do I supplement FBS with the RPMI-1640 media that is available to me? I...
13 February 2023 2,737 5 View
I’m trying to isolate only peripheral lymphocytes from whole blood using histopaque media. for that I put 3 ml blood in a 15ml tube and layer 3ml histopaque on it. Then i centrifuge it at 200g for...
13 February 2023 2,234 10 View
Anyone who has worked with culturing of lymphocytes, can u plz tell me that after I isolate the lymphocytes and put them in a CO2 incubator, I need to see if my lymphocytes have grown and whether...
18 January 2023 1,916 2 View
I’m culturing lymphocytes for MTT assay.Can someone please tell me that once I am done with the isolation stage of the lymphocytes I.e the lymphocytes have been separated from whole blood and...
14 December 2022 4,160 4 View
i am performing MTT assay using lymphocytes. as lymphocytes do not adhere to the bottom of the well, so I cannot discard the media. I intend to add my cells(suspended in RPMI-1640) in each well....
12 December 2022 6,200 3 View
I performed real-time PCR on cDNA of samples that had a nanodrop quantity of 1000+ ng/ul, but when i perform i do not get any proper results. i am attaching the report of my real-time. kindly help...
01 January 1970 8,847 4 View
I’m trying to isolate the lymphocytes using lysis buffer. That is I wash the pellet each time until I get a white pellet (the buffy coat). Then I suspend it in RPMI-1640 media. i intend to perform...
01 January 1970 3,676 2 View
Are these melt curve peaks obtained after real-time pcr alright. Kindly check attached pic?
01 January 1970 1,922 0 View
Which media is best for isolation of lymphocytes from whole blood. Histopaque-1077 (sigma) or ficol
01 January 1970 8,812 1 View
I’m trying to isolate lymphocytes from human blood using histopaque. First I added 10ml of histopaque in a 50ml falcon tube then I layer 10ml blood on top of it. The tube was centrifuged at...
01 January 1970 5,374 4 View
I have to make a solution of particulate matter of 400µg/ml in DMSO. kindly confirm, for this i need to take 400 μg of particulate matter and dissolve it in 600μg of DMSO. from this 400μg/ml stock...
01 January 1970 1,344 2 View
01 January 1970 7,855 2 View
