If my histopaque is contaminated and I want to decontaminate it, what size of syringe filter should I use? Would 0.22um syringe filter work?
0.22um filter will work to decontaminate it from microorganisms. Take in account that endotoxins can still be present in solution after filtering.
I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...
03 June 2024 4,937 0 View
Can someone guide me to an extraction method to extract polycyclic aromatic compounds, etc., from soil samples
01 April 2024 9,255 2 View
how to covert solid quantity into liquid(microlitre)?
26 March 2024 3,308 5 View
hello everyone i want to activate the 4 successissevement domains 1 then 2 then 3 and 4 on comsol multiphysics after a certain timeout t=10s? what method can i use i tried with activation under...
26 March 2024 6,435 1 View
I encountered a ZERO TOTAL READ TIME error while working with the ICP device, and I did all the things mentioned in the device's HELP, but the problem still exists, can anyone help me?
10 March 2024 4,560 1 View
I am going to calculate the root dry weight of grasses. The soil samples were collected using soil core having diameter 8.5 cm.
20 February 2024 4,937 2 View
I am an architect. Suppose I am going to design a beautiful and unique restaurant area for you so that you can eat a delicious meal next to the beautiful restaurant area. What is the most ideal...
19 February 2024 8,890 6 View
Hello ResearchGate community, I am currently exploring methods for predicting land use and land cover changes and I'm seeking recommendations for models, code libraries, and GUI software that can...
15 February 2024 6,253 2 View
Hello everyone, I'm new to using PARAFAC for decomposing EEMs data. I validated my results on three models: the first with three components, the second with four components, and the third with...
13 February 2024 791 1 View
Dear Scholars, The journal was accepted in Scopus Indexing in March 2023, and now it is available on the source page with coverage from 2019. My question is, when the journal information will be...
03 January 2024 1,151 0 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.
01 June 2024 7,419 0 View
I measured the wastewater sulfate addition with Thermo Ficher Ion Chromatography (IC) (eluent NaOH of 25 mM w/w), as I used standard solutions of sulfate in the ranges of 50 ppm, 150 ppm, 300 ppm,...
27 May 2024 9,103 5 View
We pulled our own micropipettes to extract CSF from anesthetized mice. We do not use a micrometer/syringe system, we just gently poke the micropipette tip into the appropriate location to isolate...
13 May 2024 2,632 0 View
Hi. I am currently trying to isolate Neutrophils from mouse blood. I am using histopaque 1077 and 1119. I have followed the protocol set out by Sigma, which involves layering histopaque 1119 and...
06 May 2024 9,636 2 View
Dear colleagues, as a part of our study on cyclophilins (Cyps), I wanted to test the well-known and strong interaction of CypA to cyclosporin A (CsA) on isothermal titration calorimetry (using...
23 April 2024 1,230 0 View
After cleaning your biochar, do you use filtration using GF/F filter or do you use syringe to extract the filtrate and put it on cuvette and subject it to UV-VIS? Thank you!
29 March 2024 7,918 1 View
I have experimented to assess the T-cell memory status in mice models. However, I am unable to separate the CD3+ T-cell cluster from the splenic cells using different anti-CD antibody markers,...
14 February 2024 1,769 2 View
Hello I've collected ITC data and I'm not sure if this data looks correct. This was an experiment meant to understand the properties of an enzyme thought to be active as a dimer. The sample cell...
06 February 2024 6,452 4 View
Dear all, Hello, I am recently working on isolating neutrophils from murine bone marrow by using Histopaque 1099 and histopaque 1077, but the purity of the cells are always unsatisfactory. Here I...
01 February 2024 9,203 3 View