Can anyone suggest me a list of journals in computer science discipline those go for rapid publication (monthly issue actually)?

I would like to publish some articles rapidly. It would be so nice if you suggest me a list of journals those publishes monthly usually. Thanks in advance.

30 December 2020 4,648 1 View

What is the significance of H-Index and Eigenfactor in assessing the quality of a journal?

Its getting tough now a days to successfully assess the quality of a journal. H-Index and Eigenfactor are two such parameters used frequently to determine the quality of a journal. I would like...

29 December 2020 9,032 1 View

Is there a way to isolate nuclei without purifying cytoplasmic protein aggregates?

Hello, I am trying to isolate nuclei from HEK293T cells expressing a protein that aggregates in the cytoplasm, however when I run my samples in western blot the cytoplasmic protein is strongly...

02 November 2020 9,541 3 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

Is there any complete documentation for HTM (Hierarchical Temporal Memory)?

Currently, I am working on implementing HTM for some of state of the arts problems. I am facing some issues actually. A complete documentation may help I think.

17 September 2020 8,875 1 View

The United States, China, and Thucydides Trap: Do you think there will be a war between China and the United States?

I think both countries are going to adopt solution, as the main reason for adopting the solution is obvious: nuclear deterrence - every nuclear power has the power to annihilate humanity. So it's...

07 September 2020 7,835 19 View

How to solve fog coating of glass substrate?

I'm coating metal complex on the glass substrate by using drop-casting and evaporate the solvent "DMF" on the hot plate. The glass-coated was transparent, but when the last drop of the solvent...

22 August 2020 3,616 10 View

Can someone help me find a particular article online?

Hello, I don't have access to the below article. Can anyone share it with me? Title: A Matrix Approach to Literature Reviews Authors: Salipante, Paul, William Notz, and John Bigelow Journal:...

06 August 2020 8,095 4 View

Anyone please can tell me how can I simulate carbon fibre composite plate bonded to web in simply supported steel beam by using ANSYS Apdl?

I want to simulate the CFRP plate by using ANSYS classic APDL not by using ANSYS workbench. Please help me if you have any suggestion. I used CONTA 173 associated with TARGET 170 elements as a...

04 April 2020 4,977 3 View

Why does my LCR get different readings when using different fixtures?

Dear all, I have a problem: When i measure my sample using the -Keysight 16451B Dielectric material test fixture-, the measurements (i.e the impedance outputs) are larger than the impedance...

22 March 2020 1,482 11 View

No title

03 March 2021 5,360 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

How do I avoid artifacts during paraformaldehyde fixation of mouse CD8+ T cells?

I work with mouse OT-I CD8+ T cells which have been activated in vitro with peptide-MHC and B7 proteins for 3 days. I've been trying to standardize protocols to fix these cells using...

28 January 2021 8,519 2 View

Does re-stimulation of T cells with PMA and Ionomycine before staining for intracellular cytokines for flow cytometry affect reliability of the assay?

I understand that to be able to measure intracellular cytokines there is a need to restimulate with PMA and Ionomycin but I don't understand how the results will be reliable after that. PMA and...

18 January 2021 7,240 7 View

Can someone help me to improve the MCF-10A growth?

18 January 2021 7,640 3 View