I‘m trying to culture lymphocytes. I’m attaching the pictures of the media that are available to me. kindly help me out. How do I supplement FBS with the RPMI-1640 media that is available to me? I mean in whta ratio?
Hello Mehar Khalil
You would have to supplement RPMI 1640 with 10% FBS. This means that if you would have to prepare 100ml complete RPMI 1640 media, you would add 10ml FBS to 90 ml of the RPMI 1640 media that is available to you to obtain 100ml complete RPMI1640.
Best.
I agree with Malcolm, it looks like in the photo shown, that the expiration date has passed. However, from my experience, if it has been kept refrigerated it will be fine far beyond this date.
Do you have access to the protocol regarding this specific cell type (e.g., from the producer, or from the people who was previously working with these cells)? You can also check material and methods in papers that used the same cells - just double check if they need just basal medium or supplements, depending on what you have to do with them.
Thank you for attaching the pictures of the media available to you. RPMI-1640 is a commonly used basal medium for the culture of many types of mammalian cells, including lymphocytes. Fetal bovine serum (FBS) is a common supplement for RPMI-1640 and is typically added at a concentration of 10-20%.
To supplement RPMI-1640 with FBS, you will first need to determine the volume of media you need to prepare for your experiments. Once you have this information, you can use the following formula to calculate the volume of FBS required:
Volume of FBS = (desired concentration of FBS / 100) x total volume of media
For example, if you want to prepare 100 ml of RPMI-1640 media with 10% FBS, the volume of FBS needed would be:
Volume of FBS = (10 / 100) x 100 ml = 10 ml
So you would need to add 10 ml of FBS to 90 ml of RPMI-1640 media to obtain 100 ml of RPMI-1640 media with 10% FBS.
It is important to note that the optimal concentration of FBS may vary depending on the specific type of lymphocytes you are culturing and your experimental design. You may need to perform some optimization experiments to determine the optimal concentration of FBS for your specific culture conditions.
I hope this information helps you in supplementing your RPMI-1640 media with FBS. If you have any further questions or concerns, feel free to ask.
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