Which media is best for isolation of lymphocytes from whole blood. Histopaque-1077 (sigma) or ficol
Both Histopaque-1077 (Sigma) and Ficoll are commonly used for this purpose, and each has its own advantages and disadvantages.
Histopaque-1077 is a density gradient medium that separates cells based on their density. Lymphocytes are typically less dense than other blood cells and will therefore float to the top of the gradient. Histopaque-1077 is a convenient and reliable option for isolating lymphocytes, and it is often used when high yields of pure lymphocytes are needed.
Ficoll is another type of density gradient medium that is commonly used for lymphocyte isolation. Like Histopaque-1077, Ficoll separates cells based on their density, with lymphocytes floating to the top of the gradient. Ficoll is a more widely used option compared to Histopaque-1077 and is often preferred when larger amounts of blood need to be processed.
Both Histopaque-1077 and Ficoll can be used effectively for isolating lymphocytes, and the choice between the two will depend on the specific needs of the experiment and the researcher's preferences.
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