Are these melt curve peaks obtained after real-time pcr alright. Kindly check attached pic

01 January 1970 0 2K Report

Are these melt curve peaks obtained after real-time pcr alright. Kindly check attached pic?

Badges
Science topic

More Mehar Khalil's questions See All

Can I dilute the serum sample further than stated in the manual as long as the analyte concentration remains within the KIT linear range?

I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...

03 June 2024 4,937 0 View

Can someone guide me to an extraction method to extract polycyclic aromatic compounds, etc., from soil samples ?

Can someone guide me to an extraction method to extract polycyclic aromatic compounds, etc., from soil samples

01 April 2024 9,255 2 View

How to make 50 microlitre of solution from synthesized solid nanoparticles?

how to covert solid quantity into liquid(microlitre)?

26 March 2024 3,308 5 View

Activation domain how can I?

hello everyone i want to activate the 4 successissevement domains 1 then 2 then 3 and 4 on comsol multiphysics after a certain timeout t=10s? what method can i use i tried with activation under...

26 March 2024 6,435 1 View

F31007 error ICP-OES 720 Agilent?

I encountered a ZERO TOTAL READ TIME error while working with the ICP device, and I did all the things mentioned in the device's HELP, but the problem still exists, can anyone help me?

10 March 2024 4,560 1 View

How to calculate the root biomass of grasses?

I am going to calculate the root dry weight of grasses. The soil samples were collected using soil core having diameter 8.5 cm.

20 February 2024 4,937 2 View

In your opinion, how should the best landscaping and landscape of a restaurant be? What is the most beautiful area of the restaurant in your opinion?

I am an architect. Suppose I am going to design a beautiful and unique restaurant area for you so that you can eat a delicious meal next to the beautiful restaurant area. What is the most ideal...

19 February 2024 8,890 6 View

Which specific types of models do you recommend for predicting spatiotemporal land use/land cover ?

Hello ResearchGate community, I am currently exploring methods for predicting land use and land cover changes and I'm seeking recommendations for models, code libraries, and GUI software that can...

15 February 2024 6,253 2 View

How do I choose the best model PARAFAC ???

Hello everyone, I'm new to using PARAFAC for decomposing EEMs data. I validated my results on three models: the first with three components, the second with four components, and the third with...

13 February 2024 791 1 View

When the information of the annual cite score of a new Scopus Journal usually be updated?

Dear Scholars, The journal was accepted in Scopus Indexing in March 2023, and now it is available on the source page with coverage from 2019. My question is, when the journal information will be...

03 January 2024 1,151 0 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

10 July 2024 5,985 0 View

Validated HPLC method for the estimation of the Amphotericin B ?

During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...

08 July 2024 1,330 0 View

Why there are no peaks at downfield NMR of Cobalt and Nickel complexes?

I synthesized some paramagnetic cobalt and nickel complexes (expected to be Co(II) and Ni(II)) using a bidentate ligand - N of pyridine ring (aromatic) and O of P=O (aliphatic), and I obtained...

03 July 2024 3,410 4 View

How can I put small FWHM in CasaXPS?

I'm trying to fitting Cu Auger peak into various state (Cu0, Cu+, CuI etc..) In Cu0, one of the peak's FWHM is 0.84 but when I try to enter smaller value than 0.89, "input error" is occured. How...

02 July 2024 4,932 3 View