I´ve been making a little research about resistance mechanisms in cancer and i got to this question. I thought that maybe this resistance is established by the LD50 of the drug since it would be meaningless to use high concentrations of it if the patients won´t tolerate it or the side effects will overcome the benefits.
I just want to know if there are certain parameters or guidelines to determine if a cell line is resistant to a given drug (e.g. >99% of cell viablity after being exposed to the drug, or something similar).
In our lab, we follow the protocol in the attached methods article, which includes calculating the fold-resistance. There is a table on page 7 that has cell lines and treatments with a small range of fold-resistance for each which indicates the cells are resistant. Hope this is helpful and good luck with your experiments.
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