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Questions related from Joselyn Allen
My lab is venturing into new territory by testing the affects of different antibodies on bacterial uptake/phagocytosis by macrophages. Does any one have a general phagocytosis protocol, especially...
03 December 2022 7,585 3 View
I would like to evaluate the targeting efficiency of my crispr/cas9 model in mice and would like measure the aav8 genomic copy number in different tissues including liver, heart, spleen, etc? What...
29 July 2021 851 6 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
25 February 2021 5,979 3 View
I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.
27 January 2021 8,927 2 View
I recently introduced a Lenti-Puro Guide vector containing a specific sgRNA sequence into a cell line. I would like to sequence the ddCas9-sgRNA cell lines to confirm that each sgRNA is accurate.
05 February 2019 6,796 3 View
The adipose have been sectioned and stained with H and E. Of couse F4/80 is required, however I would to crudely analyze these sections.
16 February 2016 7,074 1 View
LPS is shown to induce SREBP expression in the literature, however, there is inconsistency in the literature methods that states to perform the experiments in serum free media and other papers...
12 November 2015 9,411 3 View
I am using stimulated primary hepatocytes to detect alterations in AMPK activity. However, a paper published that serum starvation can increase AMPK activity in different cell types. Although,...
07 August 2015 3,557 3 View
I would like to assess the editing efficiency of designed guide RNAs by transfection prior to establishing a stable KO cell line (via lentiviral infection). HEK293T cells are very efficient when...
01 January 1970 8,114 2 View
Does anyone have a strategy for calculating ELISA titers from OD dilutions (without the use of standards)? The current method of my lab is through graphpad analysis (Sigmoidal 4PL, X). Using the...
01 January 1970 5,512 0 View
