Does anyone know of a mouse hepatocyte cell line that closely mimics in vivo gluconeogeneisis?

07 November 2021 0 8K Report

Hello, I would like to use a mouse cell line to study the effects of the gluconeogenesis pathway. AML12 has been mentioned to not be the best model but others have stated otherwise. I would like to study the glucagon/CREB/gluconeogenesis pathway and I would also like to validate findings with primary hepatocytes. Has anyone used AML12 to study gluconeogenesis and have seen proper induction of gluconeogenesis genes when treated?

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