The adipose have been sectioned and stained with H and E. Of couse F4/80 is required, however I would to crudely analyze these sections.
I apologize; I should have stated the name and not abbreviation but it stands for Crown Like structures.
My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...
17 June 2024 4,183 3 View
How can I study the interaction between polymer (eg PVA) and iodine computationally?
15 April 2024 3,231 2 View
Pertaining to the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Pan et. al (2023), they discussed the...
09 April 2024 4,044 1 View
I am looking for tables of elemental data (percentages of Fe, Ni, Co, Cu, etc.) for flake-like cometary dust particles like those shown below, but have not had much success finding the data. Can...
07 March 2024 1,973 0 View
These two Fe flakes are highly enriched in Ni and/or Cr. Other similar ones are composed of wustite with low oxygen. They were found in similar-aged end-glacial samples from four ocean cores in...
13 November 2023 9,364 0 View
Consider two different theories of string vibration determined by whether the string stretches as it moves. 1) If the string elongates as it bends, then the force acting on a point along the...
02 November 2023 8,845 10 View
I have been having issues with my THP-1 cells for most of this year - when I try to differentiate them with PMA into macrophage-like cells about 1/3 of the cells do not adhere properly (either...
29 October 2023 2,372 2 View
Hello, I've been doing protein purification more in the lab and I want a better understanding of the technique. I've been looking into it on my own but was wondering if anyone knew of a good...
17 October 2023 1,412 3 View
I don't think that is the intention of listing citations. Thanks.
12 September 2023 9,157 2 View
There are many common mistakes that occur in pre - analytical and post analytical phases that cause erroneous result. I want to know if there's other new research or updated research regarding...
06 September 2023 4,859 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View