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Questions related from José Avila-Peltroche
Hello everyone! I am currently working with the model organism Ectocarpus (filamentous brown algae). Our team is trying to standardize a protocol for RNA extraction using low biomass (around 10...
19 May 2024 3,968 1 View
Hello all, I am analyzing the seaweed exports from Peru during 1995-2020. In some species, exports were zero for one or two years during the abovementioned period. For calculation of Compound...
31 May 2021 9,615 4 View
Hello all, I am assessing the effect of 3 factors on cell viability after cryopreservation in some algae samples. For this, I am using a Taguchi design L18 (2^1, 3^2) and, in 2 runs, I m getting...
20 February 2020 9,099 0 View
Hello all, As a part of my thesis, I m planning to seed spores of brown seaweeds on nylon threads, so I was wondering if you can recommend me some good providers of nylon threads for seaweed...
10 December 2019 5,252 4 View
Hi all! As a part of my cryopreservation experiments, I m testing different vital stainings. I tried to check viability with FDA staining in 3 especies of filamentous brown algae (from active...
24 August 2019 2,225 4 View
Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...
21 March 2018 3,364 3 View
Hi everybody!I m culturing gametophytes from Laminariales (brown algae) in 1 L flask using PES medium. However, when I scale up these gametophytes from 500 ml to 1 L flask, in some of them...
16 March 2017 3,934 3 View
Hi again everybody :)!As a part of my research, I'm culturing some protoplast from brown algae (using non-axenic material) in different regeneration media and at different initial cellular...
28 November 2016 1,247 5 View
Hi everybody!I m working in protoplast isolation from filamentous brown algae. I have optimized the conditions and the amounts of enzymes (cellulase RS + alginate lyase) for obtaining high yields...
15 November 2016 8,118 9 View
Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....
12 July 2016 2,336 6 View
Hi everybody!I was checking some books and papers related to biostatistics and I found a no clear difference between biological replicates, replicates and repetitions (especially, between the two...
23 June 2016 6,742 13 View
Hello everybody,I am doing a research related to protoplast isolation in one species of filamentous brown algae. Now, I m testing several parameters in order to obtain high amount protoplast. The...
30 March 2016 9,043 9 View
Hi everybody,I m performing an experiment with a split plot design (1 hard to change factor and 4 easy to change factors). I was wondering how to calculate the size effects in order to report...
20 March 2016 6,056 2 View
Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...
16 February 2016 4,564 2 View
Hi everybody!I would like to ask your opinion (especially phycologist or anybody who works with plant cell suspension cultures) about this: as we know, Laminariales gametophytes (brown algae)...
06 February 2016 7,646 0 View
Hello everybody,I have prepared blue evans solutions (0.5, 0.25, 0.1 and 0.01%) in sterile seawater to asses cell viability of seaweed cells. My problem is that the final solution at any...
17 November 2015 1,256 3 View
Hello everybody!I trying to maintain samples of Gelidium elengans for protoplast isolation in sterile Petri dishes containing 100 ml of PES medium at 20°C and medium light intensity (2...
07 November 2015 1,337 8 View
Hello everybody :)My research is focus on cell suspension culture from seaweed protoplast for production of bioactive molecules. But I have a problem, I want to know if they are really in a...
24 October 2015 5,520 4 View
Hi everybody!I have collected this seaweed in the intertidial rocky shore of Ancash (north central coast of Peru). Does anybody have a suggestion about its genus or species? (I know I have to do...
10 August 2015 4,341 12 View
Hi everybody, The aim of my study is to evalute the effects of chloroformic extract of Caulerpa filiformis in motility, vitality and dna fragmentation of human sperm. The big problem is that when...
04 August 2015 2,188 8 View
I have analyzed the effect of ethanolic extracts from Caulerpa filiformis in vitality, motility and dna fragmentation of human sperm. One of the most interesting results was that concentrations of...
31 July 2015 5,061 2 View
Hi everybody, I want to determinate the asociation between change of paternity (risk factor, nominal) and the presence of pre-eclampsia (nominal) in a large sample of women. My covariate is...
08 July 2015 3,945 6 View
I have 10 morphological characters (nominal data) from a 5 seaweed species (each one with 10 specimens). I would like to perform a Cluster Analysis in SPSS but I don't know what distance measures...
16 June 2015 2,909 10 View
I started a microalgal culture in wastewater with constant illumination with an initial ph=8.5 and after 10 days the ph was 8.00. Photosynthesis tends to increase pH, so...I think other processes...
12 February 2015 2,448 12 View
Hi everybody,As a part of my thesis I calculated the growth rate of Chlorella and Chlamydomonas strains from wastewaters. Under similar conditions, Chlamydomonas had a higher growth rate than...
13 January 2015 9,692 7 View
Hi everybody, I would like to know what are the limits for phosphate and nitrate in different water bodies (lakes, river and ocean) according to international legislation (European or American). I...
06 January 2015 7,107 6 View
I have no normal data or data without homoscedasticity and I'm not very sure if this is suitable for a two way Anova analysis
22 December 2014 1,882 4 View
I have seen different ways to calculate P and N uptake rate and I don't know if there is a "standard formula" for this calculation.
19 December 2014 4,507 2 View
Hi everybody,I have a big doubt. As a part of my thesis, I am calculating the P and N uptake rate of microalgae using a linear regression, where the slope is the uptake rate. Briefly, in my linear...
18 December 2014 2,539 3 View
