Hello everybody :)
My research is focus on cell suspension culture from seaweed protoplast for production of bioactive molecules. But I have a problem, I want to know if they are really in a dedifferentiated state so I was wondering if there are any molecular marker (or markers) for this state in plants or seaweeds. I just want to be sure that the cells that I have will not form any kind of tissue and they will remain in this state.
Thank you in advance
Dear Jose,
Few papers are added for your reference.Hope you find them useful for your work!
1.An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond
Célia Miguel and Liliana Marum,Journal of Experimental Botany,2011,
2.Plants regenerated from tissue culture contain stable epigenome changes in rice Hume Stroud, Bo Ding, Stacey A Simon, Suhua Feng, Maria Bellizzi, Matteo Pellegrini, Guo-Liang Wang, Blake C Meyers, Steven E Jacobsen
3. Dedifferentiation of Arabidopsis thaliana cells is accompanied by a strong decrease in RNA polymerase II transcription activity and poly(A+) RNA and 25S rRNA eradication from the cytoplasm,Konrad Dełeńko,corresponding author Janusz Niedojadło,corresponding author Agata Łabędzka, Ewa Wiśniewska, and Elżbieta Bednarska-Kozakiewicz, Protoplasma. 2015; 252(2): 537–546. doi: 10.1007/s00709-014-0700-6
Good luck!
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