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Questions related from J. Antes
I have an experiment that supposedly increases the JNK phosphorylation. However, the phosphorylation increased from basal levels at the p54 band, the p46 band on the other hand was on a plateau in...
11 March 2021 4,341 2 View
Adding a drug causes cells to undergo oxidative stress as indicated by DCFDA fluorescence and an increase of ABCG2. However, I get inconsistent results of SOD1 after 24 hours of exposure. Also, if...
03 December 2020 3,829 5 View
We are doing a resistant cancer cell line through multiple exposures to a certain chemotherapeutic drug. Considering that the cell morphology had also changed, how would you know if the...
18 September 2020 980 4 View
We are doing an animal study to detect levels of cytokines IL—b and TNF—a at the oral alveolar bone on a rat model. Since we are currently using a new IHC kit from nichirei and abcam antibodies,...
26 November 2019 7,085 2 View
Currently, I've been working on a compound that is observed in papers to have inhibitory effect on Bcl-2 and Bax protein. However, we wanted to use the clinical concentration (higher concentration...
31 July 2019 3,345 3 View
07 July 2019 5,620 4 View
I have control and untreated groups observed through mts assay and obtained OD values for seven days. I have already plotted in the data and wanted to know how to compare both of these curves if...
01 January 2019 5,940 1 View
I have different concentrations of one compound (6 groups represents 6 kinds of concentration in a two-fold increments). All of these concentrations have the same exposure time (3 minutes). I did...
28 September 2018 5,616 3 View
09 September 2018 2,031 4 View
I prepared a 475mM Chlorhexidine+DMSO stock solution. My plan is to use this stock to dilute it to 2.375mM with medium. However, the problem was when I was adding the medium, the solution clumped...
06 August 2018 7,603 0 View
I need to test various concentrations of a compound however it is only soluble in DMSO. My compound is in pure powder form. It will be diluted again with culture medium upon exposure to cells. How...
07 July 2018 873 2 View
I'm quite new in dilutions and preparations, how can you make a 200x stock solution of any solute and solvent?
07 July 2018 3,694 4 View
Most studies using In vitro 5-ala photodynamic therapy model uses a serum-free medium while incubating with 5-ala. It is actually sensible that the presence of albumin in serum can interfere with...
01 January 1970 3,452 1 View
We are currently doing an observation on epithelial cancer cells that will be induced to EMT by multiple exposure of a certain drug. There was a decrease in e-cadherin expression while vimentin...
01 January 1970 8,213 2 View
SAS oral cancer cells have a high snail expression. We treated this cell line with a ROS inducing agent multiple times and had transformed into an EMT phenotype cell. However, we noticed that...
01 January 1970 7,356 0 View
I have two treatment modalities (Drug A and PDT) and had observed that both in combination could yield a synergistic effect but however am not sure on how to prove it through statistics. Let's say...
01 January 1970 4,182 0 View
I'm currently formulating my protocol for creating a resistant cell line against PDT. I'm planning to use a cancer cell line and expose the cells in several cycles of PDT. The cell line will be...
01 January 1970 4,447 4 View
