I'm quite new in dilutions and preparations, how can you make a 200x stock solution of any solute and solvent?
200x means 200 times more concentrated than the concentration you commonly need/use. Define this needed concentration and made a preparation 200 time more concentration as stock solution.
For example: I have this compound that needs to be dissolved in DMSO to create a stock solution first prior to diluting the solution (DMSO + compound) with media. So for a 200uL of final volume solution with the media diluted, I need to have a 0.12% concentration of the compound while at the same time reaching a 0.1% final DMSO concentration.
Let's verify if I understand your case: you need to dilute 200 times a stock solution to obtain, in diluted solution, 0.12% compound and 0.1% DMSO.
In this case the 200X stock solution must contain: 200x0.12= 24% compound, 200x0.1= 20% DMSO and 100-24-20 = 56% other solvent/media. If 200 uL is the volume of the diluted solution, mix 1 uL stock solution with 199 uL solvent/media to prepare it.
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