I need to test various concentrations of a compound however it is only soluble in DMSO. My compound is in pure powder form. It will be diluted again with culture medium upon exposure to cells. How am I going to created these concentrations: 0.96%, 0.48%, 0.12%, 0.06% (2-fold dilutions) in each series but still maintaining a 0.1% DMSO concentration?
Loredana Peca
What about making a serial dilution of 2x concentrated samples as follows: 1.92%, 0.96%, etc in water and adding at the end the same volume of
0.2% DMSO as the volume of your solution in each tube?
For example, if you have 1 ml 1.92% solution, add 1 ml 0.2% DMSO. You'll get a 0.96% solution in 1% DMSO.
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