I'm currently formulating my protocol for creating a resistant cell line against PDT. I'm planning to use a cancer cell line and expose the cells in several cycles of PDT. The cell line will be considered resistant if survival assay (MTT) will have 1.5 fold compared to the parental cells. My question is, how can I be able to test each cell generations with MTT assays if that cell generation itself must be subcultured to be exposed to PDT again. Could I make 2 plates in each generation cycle, one will be for MTT, the other one for PDT?
Thank you very much.
Hello
Kindly look at links here
http://cdn.intechopen.com/pdfs/43632/InTech-The_importance_of_cancer_cell_lines_as_in_vitro_models_in_cancer_methylome_analysis_and_anticancer_drugs_testing.pdf
https://scrc-en.tbzmed.ac.ir/uploads/91/CMS/user/file/197/books/Cancer%20cell%20culture.pdf
Good Luck
It is better to make 2 plates in each generation cycle. Or you can treat the cells with PDT with a converse time-dependent manner. For example, if you treat cells with PDT for 5 generations, you can first treat the cells for 1 generation, then treat another one. After another generetion, you treat the 3rd one. Then the 4th one, the 5th one, and so on. At the end, you get cells treated with PDT for 5, 4, 3, 2, 1 genetation, respectively. Good luck!
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