What is a good positive IHC control slide for IL—b and TNF—a?

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How to determine the consistency of cell lines after multiple treatments?

We are doing a resistant cancer cell line through multiple exposures to a certain chemotherapeutic drug. Considering that the cell morphology had also changed, how would you know if the...

17 September 2020 834 4 View

How to analyze this scenario regarding protein-protein interaction?

Currently, I've been working on a compound that is observed in papers to have inhibitory effect on Bcl-2 and Bax protein. However, we wanted to use the clinical concentration (higher concentration...

30 July 2019 3,253 3 View

How to analyze this scenario regarding protein-protein interaction?

Currently, I've been working on a compound that is observed in papers to have inhibitory effect on Bcl-2 and Bax protein. However, we wanted to use the clinical concentration (higher concentration...

06 July 2019 5,529 4 View

How to compare proliferation rate curves?

I have control and untreated groups observed through mts assay and obtained OD values for seven days. I have already plotted in the data and wanted to know how to compare both of these curves if...

31 December 2018 5,855 1 View

What kind of statistics do I need to use in this condition? ANOVA or RM ANOVA?

I have different concentrations of one compound (6 groups represents 6 kinds of concentration in a two-fold increments). All of these concentrations have the same exposure time (3 minutes). I did...

27 September 2018 5,531 3 View

What kind of statistics do I need to use in this condition? ANOVA or RM ANOVA?

I have different concentrations of one compound (6 groups represents 6 kinds of concentration in a two-fold increments). All of these concentrations have the same exposure time (3 minutes). I did...

08 September 2018 1,968 4 View

How do you create serial dilutions of a pure compound while maintaining a 0.1% DMSO concentration in each solution?

I need to test various concentrations of a compound however it is only soluble in DMSO. My compound is in pure powder form. It will be diluted again with culture medium upon exposure to cells. How...

06 July 2018 744 2 View

How do you create a 200x stock solution using Chlorhexidine and DMSO?

I'm quite new in dilutions and preparations, how can you make a 200x stock solution of any solute and solvent?

06 July 2018 3,565 4 View

Change in transwell migration unrelated to changes in EMT markers expression?

We are currently doing an observation on epithelial cancer cells that will be induced to EMT by multiple exposure of a certain drug. There was a decrease in e-cadherin expression while vimentin...

01 January 1970 8,116 2 View

Discussion on in vitro protocols of 5-ala photodynamic therapy

Most studies using In vitro 5-ala photodynamic therapy model uses a serum-free medium while incubating with 5-ala. It is actually sensible that the presence of albumin in serum can interfere with...

01 January 1970 3,345 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

Dmitry Zinovkin

Positive Control for Il-b: Skin, kidney

Positive Control for TNF-a: Human mammary cancer

Tamer A Gheita

Dear J. Antes

Check these

Article Immunohistochemical staining of tumor necrosis factor-α and ...

Article Increased TNF-α, IL-6 and decreased IL-1β immunohistochemica...

https://journals.viamedica.pl/folia_morphologica/article/view/FM.2015.0011/28987