I want to check the effect of NAC on my cells. what's the method to prepare NAC in PBS? Can i prepare it in media?
Hi Hina,
I have sent you an email with how I prepared NAC for use in cell culture. I prepared it only in cell culture medium.
N-acetyl-L-cysteine (NAC)
FW 163.2
Prep [250 mM] NAC for experimental use
1. Weigh 1 gram and dissolve in 22 ml of your choice media.
2. pH to approximately 7.3 (using 4N NaOH)
3. QS with media to have 25 ml total
4. Re-sterilize media using syringe filter.
To use NAC at [5 mM], use 98 ml of media and add 2 ml of [250 mM] NAC.
To use NAC at [10 mM], use 96 ml of media and add 4 ml of [250 mM] NAC.
Best,
Anne
Hi Hina,
I have sent you an email with how I prepared NAC for use in cell culture. I prepared it only in cell culture medium.
N-acetyl-L-cysteine (NAC)
FW 163.2
Prep [250 mM] NAC for experimental use
1. Weigh 1 gram and dissolve in 22 ml of your choice media.
2. pH to approximately 7.3 (using 4N NaOH)
3. QS with media to have 25 ml total
4. Re-sterilize media using syringe filter.
To use NAC at [5 mM], use 98 ml of media and add 2 ml of [250 mM] NAC.
To use NAC at [10 mM], use 96 ml of media and add 4 ml of [250 mM] NAC.
Best,
Anne
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