Hamidreza Safari

27 Questions 17 Answers 0 Followers

Questions related from Hamidreza Safari

I want to know that the start of the definition begins with the abstract or the introduction?

To write an article, abbreviations should be defined After that, it can be used only the abbreviation. I want to know that the start of the definition begins with the abstract or the introduction?

01 January 2019 802 3 View

I use Endnote softwar. Is there any Website or software to provide journals citation style?

The citation styles of Some journals are available, but others are not. Is there a complete source for providing journals citation styles ??? I try to obtain the citation style of Nutritional...

10 October 2018 6,820 5 View

Can I use human FTY720 drug to treat EAE model in C57 mice?

Is it FTY720 specific to humans or it can be used to treat EAE in mice as a control positive ?

03 March 2018 4,503 3 View

What is the cause of weight loss in the C57 EAE model???

I would like to know the cause weight loss of mice in theC57 EAE model.

03 March 2018 2,273 0 View

What is difference between therapeutic and prophylactic group in research ?

I want to evaluate the therapeutic and prophylactic of a drug on RA model in rat. when i should started to administration of drug in therapeutic and prophylactic group? in some studies they...

03 March 2018 4,719 5 View

How is the Stimulation index calculation?

I want to evaluate the effect of a medication on a EAE mouse model and I defined the control positive,control negative and treatment group. If i want to separate Spleen mononuclear cells and...

03 March 2018 676 2 View

Which spleen mononuclear cells in culture plate attached to the bottom plate?

In some studies to measure cytokines in the supernatant for the culture of spleen mononuclear cells, after a while, the suspensions cells are separated from sticky cells and cultivated on a...

02 February 2018 3,451 3 View

Is it possible the contamination of cells in nitrogen tank( -196C)?

I want to know if I storage the cells that were not sterile, in nitrogen tank, is it possible the growth of very low of contamination in this temperature ?

12 December 2017 7,218 1 View

I induce EAE in C57 mice and it is hard for them drinking water.How can i solve this problem?

How can i solve drinking water problem in EAE mice model?

12 December 2017 4,632 1 View

I need to find a collaborator corresponding author to write a review.How can a good corresponding author be found?

I want to write a review article about inflammation or MS, but I need a good corresponding author. How can a good corresponding author find??

12 December 2017 863 3 View

What are the complications that cause death in mice during oral gavage ?

I had one mice dead after oral gavage a plant aqueous extract after 2 weeks. i don't know what is the problem.

11 November 2017 4,614 3 View

We want to induce EAE in c57 mice, I want to know is it important mice age for inducing EAE?

I received my materials so late and mouses are older than the protocols suggested for inducing of EAE in c57 mouse

11 November 2017 3,352 3 View

I kept Interleukin 17 kit at freezing temperature for 24 hours instead of being stored at 4-8 ° C. Can I use this kit or is the kit corrupted?

The EASTBIOPHARM company recommended that kit storage at 2-8C. This mouse cytokine kit frozen for 24 hours. I want to know can i use it?

11 November 2017 6,011 4 View

Is it valuable detecting cytokine in serum?

I think cytokines range like IL-1, 6, TGF is so unstable in serum. And when blood is taken from the mice or rat, Their stress may change the range of these cytokines.

10 October 2017 6,245 3 View

Will the human beings increase their longevity with medication in the future, or will they be able to survive with the mechanization of the human bod?

Which one will have a more effective role in increasing the life span of mankind? Computer, Physics, Genetics or other things?

10 October 2017 2,777 3 View

Can I inject an aqueous extract into an intraperitoneal to mice?

The most effective compounds of my plant are flavonoids, phenols, and tannins and I don't know which of them is better, gavage or intraperitoneal

09 September 2017 2,647 8 View

Has anyone worked on a suspension cell culture that can help?

I want to use MTT test for lymphocyte cells that are the suspension, but I also need the supernatant for Eliza. Does anyone have a protocol to help? I did this by DMSO but I didn't observe any...

09 September 2017 9,621 5 View

Is it possible that aqueous extract of a plant is effective in the treatment of a disease but is not effective in preventing it?

I did this on the Wistar mouse diabetic model, and I administered rats in the prevention group on the first day and the treatment group's mice from the fifteenth day of the disease.I would like...

09 September 2017 2,200 3 View

Is there a way to understand that cells have been produced sufficiently cytokine in a medium culture that can be detected by ELISA?

I separated the spleen mononuclear cells (SMCs) from the C57 mouse spleen by using a Ficoll and seed 800,000 cells per well at volume 500 microliter and incubated for48 hours. Is the amount of the...

09 September 2017 3,978 3 View

Can i use Mitogens such as cancavelin A or phytohemagglutinin when the number of lymphocytes is low for flowcytometric test?

I separated the mononuclear cells from the spleen of the mouse and I want to measure different strains of the lymphocytes by using flow cytometric technique, but the number of cells is very low.

09 September 2017 443 3 View

Why cells that are not distributed in wells uniformly?

The cultured cells are suspensions and are not uniformly distributed in the culture medium at all.

09 September 2017 3,809 3 View

Does anyone have a good site or software for editing articles in terms of grammar and writing?

I want to edit my english article

08 August 2017 374 1 View

What is the best method for aqueous extract of plants ?

i want to extract the antiinflammatory compound of tarragon

08 August 2017 672 4 View

How can i improve Pale band by PCR setting?

I change DNA sample dose and PCR cycle but none of them was not helpful

01 January 2017 1,654 0 View

Does anyone a good LFB staining protocol for mice brain?

I want to measure brain damage in EAE mice but i dont hve any experience about that

11 November 2016 3,971 5 View

Does anyone is used Hook kit for induce EAE model? or send me a training video about use that?

I will do this in c57 bl/6 mice

10 October 2016 3,257 0 View

Hello. I'm looking for a drug that examines the effect on EAE. Please help us with the introduction of such a drug?

I have long been looking for a substance or medicine that I can work on. I will be happy with any idea

01 January 1970 216 5 View