How can i improve Pale band by PCR setting?

01 January 2017 0 2K Report

I change DNA sample dose and PCR cycle but none of them was not helpful

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More Hamidreza Safari's questions See All

I want to know that the start of the definition begins with the abstract or the introduction?

To write an article, abbreviations should be defined After that, it can be used only the abbreviation. I want to know that the start of the definition begins with the abstract or the introduction?

31 December 2018 717 3 View

I use Endnote softwar. Is there any Website or software to provide journals citation style?

The citation styles of Some journals are available, but others are not. Is there a complete source for providing journals citation styles ??? I try to obtain the citation style of Nutritional...

09 October 2018 6,724 5 View

Can I use human FTY720 drug to treat EAE model in C57 mice?

Is it FTY720 specific to humans or it can be used to treat EAE in mice as a control positive ?

02 March 2018 4,413 3 View

How is the Stimulation index calculation?

I want to evaluate the effect of a medication on a EAE mouse model and I defined the control positive,control negative and treatment group. If i want to separate Spleen mononuclear cells and...

02 March 2018 555 2 View

What is the cause of weight loss in the C57 EAE model???

I would like to know the cause weight loss of mice in theC57 EAE model.

02 March 2018 2,185 0 View

What is difference between therapeutic and prophylactic group in research ?

I want to evaluate the therapeutic and prophylactic of a drug on RA model in rat. when i should started to administration of drug in therapeutic and prophylactic group? in some studies they...

02 March 2018 4,594 5 View

Which spleen mononuclear cells in culture plate attached to the bottom plate?

In some studies to measure cytokines in the supernatant for the culture of spleen mononuclear cells, after a while, the suspensions cells are separated from sticky cells and cultivated on a...

01 February 2018 3,360 3 View

I need to find a collaborator corresponding author to write a review.How can a good corresponding author be found?

I want to write a review article about inflammation or MS, but I need a good corresponding author. How can a good corresponding author find??

11 December 2017 774 3 View

Is it possible the contamination of cells in nitrogen tank( -196C)?

I want to know if I storage the cells that were not sterile, in nitrogen tank, is it possible the growth of very low of contamination in this temperature ?

11 December 2017 7,126 1 View

I induce EAE in C57 mice and it is hard for them drinking water.How can i solve this problem?

How can i solve drinking water problem in EAE mice model?

11 December 2017 4,498 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View