How can i improve Pale band by PCR setting?

01 January 2017 0 2K Report

I change DNA sample dose and PCR cycle but none of them was not helpful

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More Hamidreza Safari's questions See All

I want to know that the start of the definition begins with the abstract or the introduction?

To write an article, abbreviations should be defined After that, it can be used only the abbreviation. I want to know that the start of the definition begins with the abstract or the introduction?

31 December 2018 840 3 View

I use Endnote softwar. Is there any Website or software to provide journals citation style?

The citation styles of Some journals are available, but others are not. Is there a complete source for providing journals citation styles ??? I try to obtain the citation style of Nutritional...

09 October 2018 6,860 5 View

Can I use human FTY720 drug to treat EAE model in C57 mice?

Is it FTY720 specific to humans or it can be used to treat EAE in mice as a control positive ?

02 March 2018 4,552 3 View

How is the Stimulation index calculation?

I want to evaluate the effect of a medication on a EAE mouse model and I defined the control positive,control negative and treatment group. If i want to separate Spleen mononuclear cells and...

02 March 2018 736 2 View

What is the cause of weight loss in the C57 EAE model???

I would like to know the cause weight loss of mice in theC57 EAE model.

02 March 2018 2,315 0 View

What is difference between therapeutic and prophylactic group in research ?

I want to evaluate the therapeutic and prophylactic of a drug on RA model in rat. when i should started to administration of drug in therapeutic and prophylactic group? in some studies they...

02 March 2018 4,767 5 View

Which spleen mononuclear cells in culture plate attached to the bottom plate?

In some studies to measure cytokines in the supernatant for the culture of spleen mononuclear cells, after a while, the suspensions cells are separated from sticky cells and cultivated on a...

01 February 2018 3,494 3 View

I need to find a collaborator corresponding author to write a review.How can a good corresponding author be found?

I want to write a review article about inflammation or MS, but I need a good corresponding author. How can a good corresponding author find??

11 December 2017 909 3 View

Is it possible the contamination of cells in nitrogen tank( -196C)?

I want to know if I storage the cells that were not sterile, in nitrogen tank, is it possible the growth of very low of contamination in this temperature ?

11 December 2017 7,256 1 View

I induce EAE in C57 mice and it is hard for them drinking water.How can i solve this problem?

How can i solve drinking water problem in EAE mice model?

11 December 2017 4,678 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View