Does anyone a good LFB staining protocol for mice brain?

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Adejoke Yetunde Onaolapo

Find below staining protocol for Luxol fast blue, any of the two will do

1. web.qbi.uq.edu.au/microscopy/wp-content/...to.../luxol-fast-blue.pdf

2. https://www.uq.edu.au/.../Luxol%20Fast%20blue%20Staining%20Proced..

I hope it helps

Zohre Salehabadi

Rasool Zeidabadi

PhD

assistant professor

Hakim Sabzevari University, Sabzevar · department of physical education and sport science

He has phd thesis about rats. I hope its be usefull.

Polina Shkorbatova

I can send you LFB protocols on paraffin or frosen sections, as you prefere. It worked in rat and cat brain. But I am really curious how you will quantify the brain damage. And why don't you try Myelin Basic Protein Staining? LFB stains not only myelin. MBP is more specific

https://www.ncbi.nlm.nih.gov/pubmed/11819099

here is a good method of LFB staining of frozen tissue

I used it several times