I think cytokines range like IL-1, 6, TGF is so unstable in serum. And when blood is taken from the mice or rat, Their stress may change the range of these cytokines.
Most innate cells cytokines can be released during clotting and do not use serum but plasma is highly recommended or use heparinized tubes to avoid such challenge. Good Luck!
Dear Dr. Hamidreza Safari
Cytokines are critical mediators of both the innate and the adaptive immune responses. Detection of the spectrum of inflammatory and anti-inflammatory cytokines has, therefore, become increasingly important to understand the pathogenesis and diagnosis of several diseases. Moreover, in the absence of tools to routinely assess immune responses measurements of cytokines and other inflammatory mediators or markers can provide predictive clinical information.
In recent years, commercially available multiplex technologies which detect large numbers of proteins in small volumes of a sample have provided investigators with opportunities to begin addressing the complexity of inflammatory responses. Nevertheless, careful attention to conceptual and methodological considerations is essential, as they can significantly impact the reliability and reproducibility of cytokine detection, especially when using novel technologies.
Circulating levels of cytokines are measured from plasma or serum collected from peripheral blood draws. It is important to note that the methods of sampling and storage of plasma or serum are critical for accurate measurements.
Some useful references:
Standard operating procedures for serum and plasma collection: early detection research network consensus statement standard operating procedure integration working group. M.K. Tuck, D.W. Chan, D. Chia, A.K. Godwin, W.E. Grizzle, K.E. Krueger, et al., J. Proteome Res. 8 (2009) 113–117. doi: 10.1021/pr800545q. https://www.ncbi.nlm.nih.gov/pubmed/19072545
Prerequisites for cytokine measurements in clinical trials with multiplex immunoassays. de Jager W, Bourcier K, Rijkers GT, Prakken BJ, Seyfert-Margolis V. BMC Immunol. 2009 Sep 28;10:52. doi: 10.1186/1471-2172-10-52. https://www.ncbi.nlm.nih.gov/pubmed/19785746
Kind regards,
Jesus Eduardo Elizondo
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